This blog is devoted to BIOL 6988, a graduate level seminar in the biological sciences at Youngstown State University. While targeted towards graduate students, BIOL 6988 actively incorporates undergraduate participants in their scholastic endeavors in the biological sciences. This blog is intended as a educational tool not just for YSU students and faculty, but for anyone who wishes to contribute to an active-learning environment.
Good Job Kory! Neurospora crassa has played a great role in the development of Modern genetics and from looking at the background information,I found that this fungi utilize glucose as a carbon source.What factors do you think would be involved to avoid the repression of the qa genes in Neurospora crassa even at the presence of glucose?
To avoid repression of the qa genes in the presence of glucose an inducer would have to bind to the qa-1S repressor protein. If an inducer is not present, then the qa genes will continue to be repressed in the presence of a favorable carbon source such as glucose. In the case of quinic acid acting as the inducer, if a preferred carbon source is present then catabolite repression of qa permease will occur preventing an increase in the concentration of quinic acid leading to the repression of the qa genes.
Very well done Kory, nice presentation style! Sort of completely unrelated to the above-posed question, but looking up a few things, I just saw that there is an annual scientific conference dedicated to Neurospora.. with committees and dinners and presentations and everything.. In a really nice part of California.. It was just held March 10-13. Did anyone have knowledge of this? We should take a field trip..
Very nice presentation, Kory! Since Zac throughly answered the question, I have to agree with Jillian and say that we should take a field trip! It's also pretty neat that there is a conference held just for Neurospora!
I thought the data pointing to oscilations in expression was interesting. How important would a circadian cycle of expression be for Neurospora crassa?
As everyone has said, Kory did a great job with his presentation! Amazing to think that a simple mold discovered in a bakery 170 years ago has yielded so many advancements in scientific knowledge. It goes to show that discoveries are often made in unlikely places.
Great presentation Kory. I agree with Zac. If you want to avoid repressing the quinic acid gene cluster, then you would need an inducer in order to disable the repressor in the primary system of gene control.
Good job Kory! I agree that we should try to get together and go take this field trip next time it comes around. On a separate note, I agree with Zac and Michelle on the repression of the qa gene cluster. I also found it interesting that although the qa-1s protein isn't known, many studies have failed to show the binding of the repressor to DNA. I agree with the paper about the fact that it may indirectly indicate that the target may be an activator protein.
Great job Kory! I agree with Zac and Michelle about the repression aspect, with an added note about the inducer: if you want the inducer to affect only the quinic acid genes, could you isolate the genes and put them on some sort of controllable plasmid (or something similar) in order to have the inducer be a bit more out of the norm so that it doesn't also accidentally induce other gene clusters?
Great job Kory! I agree with Joseph in wondering the same question. although I am not familiar with this subject I did enjoy his research and learned a lot from it
Good Job Kory!
ReplyDeleteNeurospora crassa has played a great role in the development of Modern genetics and from looking at the background information,I found that this fungi utilize glucose as a carbon source.What factors do you think would be involved to avoid the repression of the qa genes in Neurospora crassa even at the presence of glucose?
To avoid repression of the qa genes in the presence of glucose an inducer would have to bind to the qa-1S repressor protein. If an inducer is not present, then the qa genes will continue to be repressed in the presence of a favorable carbon source such as glucose. In the case of quinic acid acting as the inducer, if a preferred carbon source is present then catabolite repression of qa permease will occur preventing an increase in the concentration of quinic acid leading to the repression of the qa genes.
ReplyDeleteVery well done Kory, nice presentation style! Sort of completely unrelated to the above-posed question, but looking up a few things, I just saw that there is an annual scientific conference dedicated to Neurospora.. with committees and dinners and presentations and everything.. In a really nice part of California.. It was just held March 10-13. Did anyone have knowledge of this? We should take a field trip..
ReplyDeleteVery nice presentation, Kory! Since Zac throughly answered the question, I have to agree with Jillian and say that we should take a field trip! It's also pretty neat that there is a conference held just for Neurospora!
ReplyDeleteI thought the data pointing to oscilations in expression was interesting. How important would a circadian cycle of expression be for Neurospora crassa?
ReplyDeleteAs everyone has said, Kory did a great job with his presentation! Amazing to think that a simple mold discovered in a bakery 170 years ago has yielded so many advancements in scientific knowledge. It goes to show that discoveries are often made in unlikely places.
ReplyDeleteAwesome job Kory! You did a great job explaining your goals and what was needed to be completed to obtained them. Made the topic very understandable.
ReplyDeleteGreat presentation Kory. I agree with Zac. If you want to avoid repressing the quinic acid gene cluster, then you would need an inducer in order to disable the repressor in the primary system of gene control.
ReplyDeleteGood job Kory! I agree that we should try to get together and go take this field trip next time it comes around. On a separate note, I agree with Zac and Michelle on the repression of the qa gene cluster. I also found it interesting that although the qa-1s protein isn't known, many studies have failed to show the binding of the repressor to DNA. I agree with the paper about the fact that it may indirectly indicate that the target may be an activator protein.
ReplyDeleteGreat job Kory! I agree with Zac and Michelle about the repression aspect, with an added note about the inducer: if you want the inducer to affect only the quinic acid genes, could you isolate the genes and put them on some sort of controllable plasmid (or something similar) in order to have the inducer be a bit more out of the norm so that it doesn't also accidentally induce other gene clusters?
ReplyDeleteGreat job Kory! I agree with Joseph in wondering the same question. although I am not familiar with this subject I did enjoy his research and learned a lot from it
ReplyDelete