Bill Rees To Present Thesis Defense
The affable and diligent young man who goes by the moniker "Bill Rees" will be presenting his research results as part of his thesis defense during seminar on April 8th. Bill's research should be very topical, so be sure to come out and support him!
Awesome job Bill, #teamfagan! I find the possible applications of phage display to be very interesting. What other possible applications could use phage display technology?
ReplyDeleteGreat JOB… Bill !! I have found some other important applications of Phage Display Technology which are on gene delivery and tumor targeting. The gene therapy vector can be modified by selecting alternative ligands which bind to cell surface receptors for targeting viral and non-viral vectors. As a result, it increases their selectivity, efficacy and reduce toxicity of gene delivery into mammalian cells. Phage display has been used for the delivery of cytotoxic chemotherapy.
ReplyDeleteI found the use of phage display technology in targeting gp120 of HIV-1 particularly interesting. Through the application of this process virologists have been able to distinguish immunodominant regions and Fab binding sites that bind Ag with high affinity. Using this information, antiviral compounds that specifically bind to these Fab sites could then be synthetically manufactured.
ReplyDeleteCancer-germline genes, such as MAGE, which are expressed in many tumors but not in normal tissues. The only normal cells in which significant expression of such genes that has been detected are placental trophoblasts and testicular germ cells. Because these cells do not express MHC class I molecules, gene expression should not result in expression of the antigenic peptides and such antigens can therefore be considered as strictly tumor-specific
ReplyDeleteDifferentiation antigens, are also expressed in the normal tissue of origin of the malignancy. The paradigm is tyrosinase, which is expressed in normal melanocytes and in most melanomas. Antigens of this group are not tumor-specific, and their use as targets for cancer immunotherapy may result in autoimmunity towards the corresponding normal tissue. In the case of melanocytes, the risk of inducing severe side effects appears minimal, and could be limited to the appearance of vitiligo. More serious concerns about autoimmune side effects apply to carcinoembryonic antigen (CEA), an oncofetal protein expressed in normal colon epithelium and in most gut carcinomas. Autoimmune toxicity should not be an issue, however, in situations where the tissue expressing the antigen is dispensable or even resected by the surgeon in the course of cancer therapy, as would be the case for prostate specific antigen (PSA).
I was wondering if you know why or how phage display became a lab technique?
ReplyDeleteI read an article on the use of phage display to regulate the antiangiogenic factors in human vein endothelial cells via a specific kinase domain receptor. The goal of the protocol was to block the KDR's interaction with VEGF and it yielded 70% reduced tumor growth in mice. It goes without saying that phage display is very useful and Bill did an excellent job in his presentation!
ReplyDeleteLet me first echo everyone else by saying that you did a great job! It will be interesting to see what factors are causing the differential binding between whole blood and pure HSA. Jillian raises an interesting avenue of thought by bringing up the potential for specified binding as a means of blocking receptor sites. I'm sure there are numerous other situations in which such a method could be used to target specific cell types.
ReplyDeleteGreat job!! In other studies, I have read that phage display is being used to isolate diverse antibodies. I have also read it can be a possibility with bioplanning. This is used to enrich the phage clones with binding affinities for a specific target.
ReplyDeleteI think I may have read the same article as Jillian, because I saw a similar reporting of phage display being used to block VEGF interaction leading to drastically reduced tumor growth. That said, it brings an interesting thought of using this method to block other receptors.
ReplyDeleteI just noticed that Marshall brought up the same thing. Glad to see someone else is on the same thought train as me.
Phage-displayed peptide libraries can be used for B-cell and T-cell epitope mapping, the selection of bioactive peptides bound to receptors or proteins, disease-specific antigen mimics, peptides bound to non-protein targets, cell-specific peptides, or organ-specific peptides, and the development of peptide-mediated drug delivery systems and other applications. Phage display technology has also been applied as a tool for directed evolution and can be applied for mechanistic-based studies and to generate enzyme variants with new or improved properties.
ReplyDeleteJust like everyone else said, Great job! I was wondering if there was a list of what a phage display can show other then this research and if there was more research that can be done with phage displaying
ReplyDelete