Due to the Thanksgiving holiday, there will be no seminar this week (Friday, November 25th).
However, Mr. Mike Kelly will be the final presenter for this semester on Friday, December 2nd. The title of his talk shall be forthcoming.
There is NO SEMINAR on the final Friday of the semester (December 9th).
UPDATE: (11/29/16)
Mike's presentation title is "Mesenchymal Stem Cell influence on Fibroblast Collagen Synthesis". Background reading can be found at the following URL:
Mike’s presentation on mesenchymal stem cell influence on fibroblast collagen synthesis provided valuable insight to research regarding regenerative medicine. Mike did an excellent job providing background information regarding his thesis. It was interesting to hear about the phases of wound healing and the roll of mesenchymal stem cells in this process as well as the prior research studies that have been done at YSU. I found it particularly interesting that in “group 3” of a prior study, a wound in a mouse showed double the tensile strength after four weeks healing time (in the presence of mesenchymal stem cells, PRP, and CollaTape) than to normal healing after eight weeks in the control group (“group 1”). This shows then that there is obvious benefit from utilizing this method in wound healing. My question then is what are some possible downsides or adverse effect of using mesenchymal stem cells for wound healing?
ReplyDeleteAlthough the MSCs obviously have some positive effects on wound healing. A downside is that their actual effect is still unknown and they don't know where they go after they are done having their effect. Also, cost and they must be administered during surgery.
ReplyDeleteMike’s research on MSC contribution to wound healing has obvious benefits. For example, if MSCs cause wounds to heal better by increasing collagen content at the wound sight, a post-surgery MSC application at the incision may help to reduce the risk of wound dehiscence. This is definitely a good thing, but this kind of treatment does have its downsides. As Nina mentioned, this type of treatment may be costly. I imagine that MSC sources are rather limited, and if MSC wound treatment is successful, then supply and demand will take over and costs will go up. Additionally, because MSCs are capable of differentiating into multiple cell types depending on which growth factors and signals are nearby, there is a risk that an MSC treatment differentiates into the wrong type of cell and become tumorous. This concern especially must be addressed prior to green lighting any sort of MSC clinical treatment.
ReplyDeleteI think that Mike did really well on his presentation last Friday and I found the current results to be interesting. I have only really heard about the advantages of MSCs. However, I read two articles that discuss the disadvantages/limitations of MSCs (which I have attached the link to). While the potential role of MSCs to influence tissue engineering is undeniable, there are limitations that result from using MSCs. Although rare, in vivo fusion of MSCs to endogenous differentiated cells has been observed. Furthermore, MSCs have displayed inhibitory effects on T-cell proliferation. They secrete high levels of interleukin-6 and VEGF, as well as down regulate MHC class II, CD40 and CD86 molecules on dendritic cells, all of which are directly correlated to the inhibition of T-cell proliferation. One study determined that MSCs secrete cytokines and other factors that suppress the immune response in addition to inhibiting apoptosis and fibrosis. Similarly, MSCs have also been shown to foster tumor growth in allogenic recipients. Additionally, a review that looked at the use of MSCs in regeneration of articular cartilage and intervertebral disc engineering found that the physiological microenvironment of the degenerate joint or spine is likely to be hypoxic, acidic, deprived of nutrients, and exposed to higher than normal concentrations of pro-inflammatory cytokines and reactive oxygen species which would not be favorable for MSCs. Overall, I think that MSCs can have really beneficial effects but we just have to figure out the limitations and try to correct them when possible.
ReplyDeletehttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2634175/
https://www.ncbi.nlm.nih.gov/pubmed/19725073
Mike's presentation was done very well and I really enjoyed the topic. I had also only previously heard about the advantages of using MSC sources for wound treatment. And while it may be a useful method, it has also been pointed out that cost is one of the biggest factors here. I also thought Marissa's comment was useful in examining the downfalls of this methods in terms of its limitations. MSC use as wound healing is something that can definitely be beneficial in the future but needs to be studied more.
ReplyDeleteI found Mike's presentation to be very interesting. I have read articles about mesenchymal stem cells and their potential in wound-healing but it is awesome to hear the research first-hand from a fellow YSU grad student. While mesenchymal stem cells are attractive due to their regenerative capabilities, every treatment has potential risks associated with it. One possible downfall, as previously discussed is cost. Isolation of mesenchymal stem cells requires surgery, which is not only expensive but carries its own risks such as infection and internal bleeding. I remember reading a scholarly article for a class that also discussed the immunosuppressive effects of mesenchymal stem cells and their potential to mediate infection at the injection site. Lastly, there could also be the risk of tumor development if the stem cells don't respond how they are desired to in their new environment.
ReplyDeleteI found his presentation on wound healing to be very interesting. Potential downfalls for MSC useage would be cost and not being have enough research being done on the effects of it overtime. One possibility is to study the long term effects of MSC on a group of cells and determine how it interacts with the surrounding cells and its overall functionality in wound healing. Use of MSC has great potential, but I think more research needs to be done on its effects.
ReplyDeletemike kelly presentation was interesting and aspiring to me since it emphasized on MSC regeneration, leading to wound healing. Despite its application on surgery, MSC has its own downside that may limit its application.firstly, i think MSC application may not be cost-effective and may be used by few people.lastly, i think more investigation need to be done on MSC application in the body not to cause any detrimental effect on the cells instead of healing.
ReplyDeleteI found Mike's presentation very interesting and he did a good job in giving out necessary background information. It was interesting to know that BM-MSCs have anti-inflammatory effects (Zhao, et al), increased fibrin contraction and local strength (Fathke, et.al) which all played a part in improved fascial healing of rats. I would be interested to know the volume of BM-MSCs required per centimeter square area of the wound, if this approach is applied to humans and what would be the cost of it?
ReplyDeletehttps://www.ncbi.nlm.nih.gov/pubmed/19824807?dopt=Abstract
https://www.ncbi.nlm.nih.gov/pubmed/15342945?dopt=Abstract
A downside could be obtaining the MSCs during surgery. I'm not familiar with the exact process, but I know that it requires patient consent and lengthens the total time of the surgery. With that said, I am unaware of safer alternatives to obtain MSCs.
ReplyDeleteMike had an excellent presentation, very professional and informing. He did a great job explaining the background of mesenchymal stem cells and their effects in the wound healing process. It was very evident that MSCs are a promising option due to their low immunogenicity and immunosuppressive and tissue repair capabilities. But disadvantages including cost, tumorigenic potential, etc. as mentioned by my peers, do exist. Further investigations are needed to enhance the consistency and efficacy of MSCs when used in tissue repair.
ReplyDelete