This blog is devoted to BIOL 6988, a graduate level seminar in the biological sciences at Youngstown State University. While targeted towards graduate students, BIOL 6988 actively incorporates undergraduate participants in their scholastic endeavors in the biological sciences. This blog is intended as a educational tool not just for YSU students and faculty, but for anyone who wishes to contribute to an active-learning environment.
Last Friday, Thywill did a wonderful job presenting Achilles tendon repair in rats using platelet-rich plasma and bone marrow derived from mesenchymal stem cells. Working with orthopedic surgeons and physical therapists, in addition to studying biomechanics, I found her talk particularly interesting. Besides the typical surgical and non-surgical method to help healing, new alternatives are being investigated. These methods include: collagen dressings, platelet-rich plasma (PRP) that includes numerous growth factors, and bone marrow mesenchymal stem cells (BM-MSCs). Thywill presented the papers with studies already done that correlate to her research in which she is combining the methods and comparing them based upon highest healing capacity and fastest healing capacity in relation to Achilles tendon repair. Four treatment groups were formed: Group 1 used collagen only, Group 2 used PRP and collagen, Group 3 used MSC and collagen, and Group 4 used collagen, PRP, and MSC. Based on the different healing factors that each alternative possess, which treatment group would be expected to have the fastest healing rate?
One point I found interesting during Thywill's presentation was the association of renal transplants with Achilles tendon damage, an association I would not think of, but is informative of underlying causes of Achilles tendon damage. Ideally Thywill's research will find a method of non-surgical repair that will show results as good as surgical repair. Clearly her research has involved plenty of background work to prepare for the bio mechanical testing. This includes the BM-MSC isolation and the differential assay done to test their ability to differentiate. Group 4 treated with collagen PRP, and the MSC I'd expect to show the best results. I would not think that any of those conditions would act against another. In addition to the benefits provided by collagen, the addition of PRP or MSC provides growth factors critical in the healing process. Between Group 2 and Group 3, I suspect the MSC treatment will show better results, but am curious which will.
Out of the four treatment groups, I would expect the treatment given to group four, using collagen, PRP, and BM-MSCs, to have the fastest healing rate due to the inclusion of multiple factors promoting the healing process. I would expect group one, using only collagen, to be the slowest healing of the four groups. Between group two, including the collagen and PRP, and group three, including the MSC and collagen, I would expect to to see healing rates on the spectrum between group one and group four. I would also hypothesize that group three's inclusion of MSCs will have a faster healing rate than group two's inclusion of PRP. I would be very interested in seeing the results when the study is done.
I thought Thywill did a great job presenting her research. She gave a lot of background information that helped explain her study to the audience. I think her results will be very interesting when she is completed her testing. I also think that Group 4, PRP and MSC, will have the fastest healing rate. Combined with collagen, these give out growth factors that promote healing and with more of these present it would be expected that it would heal at a faster rate. The other groups having only one of these combined with collagen or just collagen alone would heal slower due to fewer of these growth factors being present. I am excited for Thywill's study and what her results will be.
Out of the several groups that thywill described I would say that group 4, followed by group 3 would provide the best healing environment. I believe it is the combination of stem cells with the support of collagen that would provide the much needed scaffolding of collagen and the regenerative properties of stem cells to induce accelerated healing..
Hello all! Although, I was not at seminar due to my final interview at the University of Toledo, I know a lot of the details about this project, since I am in the same lab as Thywill. I would have to agree with the majority of people here when it comes to this question. Group 4, may have an advantage due to the different components contributing to the healing process. However, I am interested in seeing how much the PRP affects the wound healing and healing rates of the calcaneal tendon, and how group 4 compares to group 3.
Thywill presentation was interesting and enjoyable. She had in-depth knowledge about her thesis study research. The addition of collagen to the mostly researched mesenchymal stem cells and platelets rich plasma in response to achilles tendon repair will yield better results. I concur with everyone that group 4 will have an advantage towards the healing process due to the addition of collagen. Also it is a clever approach to do bio-mechanical testing to measure tensile strength of the repaired achilles tendon.
Thywill's work is fascinating to me. I really enjoy that it shows the application and importance of the aspects we learn as biology undergraduate students. I agree with my peers that Group 4 will have the best healing results. I agree with Nathan that Group 3 with the MSC treatment will most likely work better than the PRP utilized in Group 2. I am interested in seeing how the results of Groups 2 and 3 differ. The biomechanical aspect of Thywill's research is intriguing. Her project truly comes full circle in evaluating the best treatment options and their effect on the mobility and strength of the calcaneal tendon post-repair. Awesome job Thywill!
Last Friday, Thywill did a wonderful job presenting Achilles tendon repair in rats using platelet-rich plasma and bone marrow derived from mesenchymal stem cells. Working with orthopedic surgeons and physical therapists, in addition to studying biomechanics, I found her talk particularly interesting.
ReplyDeleteBesides the typical surgical and non-surgical method to help healing, new alternatives are being investigated. These methods include: collagen dressings, platelet-rich plasma (PRP) that includes numerous growth factors, and bone marrow mesenchymal stem cells (BM-MSCs). Thywill presented the papers with studies already done that correlate to her research in which she is combining the methods and comparing them based upon highest healing capacity and fastest healing capacity in relation to Achilles tendon repair. Four treatment groups were formed: Group 1 used collagen only, Group 2 used PRP and collagen, Group 3 used MSC and collagen, and Group 4 used collagen, PRP, and MSC. Based on the different healing factors that each alternative possess, which treatment group would be expected to have the fastest healing rate?
One point I found interesting during Thywill's presentation was the association of renal transplants with Achilles tendon damage, an association I would not think of, but is informative of underlying causes of Achilles tendon damage. Ideally Thywill's research will find a method of non-surgical repair that will show results as good as surgical repair. Clearly her research has involved plenty of background work to prepare for the bio mechanical testing. This includes the BM-MSC isolation and the differential assay done to test their ability to differentiate.
ReplyDeleteGroup 4 treated with collagen PRP, and the MSC I'd expect to show the best results. I would not think that any of those conditions would act against another. In addition to the benefits provided by collagen, the addition of PRP or MSC provides growth factors critical in the healing process. Between Group 2 and Group 3, I suspect the MSC treatment will show better results, but am curious which will.
Out of the four treatment groups, I would expect the treatment given to group four, using collagen, PRP, and BM-MSCs, to have the fastest healing rate due to the inclusion of multiple factors promoting the healing process. I would expect group one, using only collagen, to be the slowest healing of the four groups. Between group two, including the collagen and PRP, and group three, including the MSC and collagen, I would expect to to see healing rates on the spectrum between group one and group four. I would also hypothesize that group three's inclusion of MSCs will have a faster healing rate than group two's inclusion of PRP. I would be very interested in seeing the results when the study is done.
ReplyDelete^^ Anna Vietmeier
DeleteI thought Thywill did a great job presenting her research. She gave a lot of background information that helped explain her study to the audience. I think her results will be very interesting when she is completed her testing.
ReplyDeleteI also think that Group 4, PRP and MSC, will have the fastest healing rate. Combined with collagen, these give out growth factors that promote healing and with more of these present it would be expected that it would heal at a faster rate. The other groups having only one of these combined with collagen or just collagen alone would heal slower due to fewer of these growth factors being present.
I am excited for Thywill's study and what her results will be.
Out of the several groups that thywill described I would say that group 4, followed by group 3 would provide the best healing environment. I believe it is the combination of stem cells with the support of collagen that would provide the much needed scaffolding of collagen and the regenerative properties of stem cells to induce accelerated healing..
ReplyDeleteHello all! Although, I was not at seminar due to my final interview at the University of Toledo, I know a lot of the details about this project, since I am in the same lab as Thywill. I would have to agree with the majority of people here when it comes to this question. Group 4, may have an advantage due to the different components contributing to the healing process. However, I am interested in seeing how much the PRP affects the wound healing and healing rates of the calcaneal tendon, and how group 4 compares to group 3.
ReplyDeleteThywill presentation was interesting and enjoyable. She had in-depth knowledge about her thesis study research. The addition of collagen to the mostly researched mesenchymal stem cells and platelets rich plasma in response to achilles tendon repair will yield better results. I concur with everyone that group 4 will have an advantage towards the healing process due to the addition of collagen. Also it is a clever approach to do bio-mechanical testing to measure tensile strength of the repaired achilles tendon.
ReplyDeleteThywill's work is fascinating to me. I really enjoy that it shows the application and importance of the aspects we learn as biology undergraduate students. I agree with my peers that Group 4 will have the best healing results. I agree with Nathan that Group 3 with the MSC treatment will most likely work better than the PRP utilized in Group 2. I am interested in seeing how the results of Groups 2 and 3 differ. The biomechanical aspect of Thywill's research is intriguing. Her project truly comes full circle in evaluating the best treatment options and their effect on the mobility and strength of the calcaneal tendon post-repair. Awesome job Thywill!
ReplyDelete