This blog is devoted to BIOL 6988, a graduate level seminar in the biological sciences at Youngstown State University. While targeted towards graduate students, BIOL 6988 actively incorporates undergraduate participants in their scholastic endeavors in the biological sciences. This blog is intended as a educational tool not just for YSU students and faculty, but for anyone who wishes to contribute to an active-learning environment.
I really enjoyed Dan's presentation and it was very interesting. He talked how mesenchymal stromal cells (MSCs), platelet-rich plasma (PRP) and collagen could have healing effect on injured achilles tendon of rat. MSCs are adherent cells by nature, prp promotes clumping of cells and collagen has great tensile strength and these biologics, from Dan's presentation, are hypothesized to promote healing of the achilles tendon.
My question is, will omitting one of the biologics increase or decrease wound healing of the rat's achilles tendon? And why?
Dan did an excellent job in his presentation. In my personal opinion, omitting one of the biologics will decrease wound healing. MSCs help in wound healing through modulating the inflammatory environment by regulating the activities of the resident macrophages to enhance tendon healing. PRP augments and accelerates tendon healing by supplying abundant growth factors contained in platelets and fibrin matrix which functions as a natural conducive scaffold to facilitate tissue healing. Collagen is the major extracellular matrix protein with a chemotactic role where it attracts fibroblast and encourages deposition of new collagen to the wound bed. Therefore, treatment of tendons with PRP can increase production of some collagen types and also increase the number of cells. PRP induces differentiation of MSCS into tenocytes which proliferates to produce abundant collagen. These three biologics work cooperatively towards wound healing thus omission of one will mean wound healing won't be attained.
I do agree with Annah on this that omitting one of the biologics would decrease the healing process. Dan talked about the Mesenchymal stromal cells, Plasma-rich plasma and the Colla tape which in this case each has its own mechanism of healing. Do you think using the three biologics, two or one would strengthen the tendon and avoid re-rupturing? If you were to choose two of the biologics which combination would be the best and why? Which of the three biologics do you think is the most preferred in would healing and why?
Great job Dan! Answering Patricia’s question, I believe that using all 3 of the biologics will more than likely be the best approach to strengthen the tendon. If one of the biologics had to be removed though, according to the normalized data, it should be the PRP. This means that the collagen and MSC would be the optimal combination. With that being said, collagen should be the best biologic to use solo; but, upon further questioning Dan, all of the surgical control data would need to be done (each biologic alone and then combinations of two) to concretely prove my statement as either true or false.
Awesome job Dan on the informative presentation. Along with Annah and Patricia, omitting one of the biologics would most likely decrease the healing process. When using all three together you are basically "throwing gasoline to the fire" to speed the healing time. All three of these biologics are important and are currently used in wound healing. When it comes to choosing which biologic to use, it may be based on a financial standpoint or physicians preference.
There is never one answer to a problem in biology and I think this presentation showed that. Several factors affected healing and by the use of all of them were you able to obtain optimal healing. Overall very nice presentation and appreciate for the surgical warnings!
Great job Dan, to answer the questions presented earlier in the comments, I believe that all 3 of the biologics are designed to work together to promote healing. This would be the ideal situation to correct an achilles tendon tear; However, if one had to be omitted, I tend to agree with Dakota's point. Based on the data, PRP would be the best to be removed leaving collagen and MSC. I am curious to see that if one biologics is removed, if wound healing would halt, or just be slowed.
Eliminating one of the biologics would probably decrease wound healing since his data was comparing the biologics to no biologics. This is a really cool study since achilles tendon injuries are common and take a long time to heal leaving the person to get more atrophy and more physical therapy needed for rehabilitation after the injury.
I really enjoyed Dan's presentation and it was very interesting. He talked how mesenchymal stromal cells (MSCs), platelet-rich plasma (PRP) and collagen could have healing effect on injured achilles tendon of rat. MSCs are adherent cells by nature, prp promotes clumping of cells and collagen has great tensile strength and these biologics, from Dan's presentation, are hypothesized to promote healing of the achilles tendon.
ReplyDeleteMy question is, will omitting one of the biologics increase or decrease wound healing of the rat's achilles tendon? And why?
Dan did an excellent job in his presentation. In my personal opinion, omitting one of the biologics will decrease wound healing. MSCs help in wound healing through modulating the inflammatory environment by regulating the activities of the resident macrophages to enhance tendon healing. PRP augments and accelerates tendon healing by supplying abundant growth factors contained in platelets and fibrin matrix which functions as a natural conducive scaffold to facilitate tissue healing. Collagen is the major extracellular matrix protein with a chemotactic role where it attracts fibroblast and encourages deposition of new collagen to the wound bed. Therefore, treatment of tendons with PRP can increase production of some collagen types and also increase the number of cells. PRP induces differentiation of MSCS into tenocytes which proliferates to produce abundant collagen. These three biologics work cooperatively towards wound healing thus omission of one will mean wound healing won't be attained.
ReplyDeleteI do agree with Annah on this that omitting one of the biologics would decrease the healing process. Dan talked about the Mesenchymal stromal cells, Plasma-rich plasma and the Colla tape which in this case each has its own mechanism of healing. Do you think using the three biologics, two or one would strengthen the tendon and avoid re-rupturing? If you were to choose two of the biologics which combination would be the best and why? Which of the three biologics do you think is the most preferred in would healing and why?
ReplyDeleteThis comment has been removed by the author.
ReplyDeleteGreat job Dan! Answering Patricia’s question, I believe that using all 3 of the biologics will more than likely be the best approach to strengthen the tendon. If one of the biologics had to be removed though, according to the normalized data, it should be the PRP. This means that the collagen and MSC would be the optimal combination. With that being said, collagen should be the best biologic to use solo; but, upon further questioning Dan, all of the surgical control data would need to be done (each biologic alone and then combinations of two) to concretely prove my statement as either true or false.
DeleteAwesome job Dan on the informative presentation. Along with Annah and Patricia, omitting one of the biologics would most likely decrease the healing process. When using all three together you are basically "throwing gasoline to the fire" to speed the healing time. All three of these biologics are important and are currently used in wound healing. When it comes to choosing which biologic to use, it may be based on a financial standpoint or physicians preference.
ReplyDeleteThere is never one answer to a problem in biology and I think this presentation showed that. Several factors affected healing and by the use of all of them were you able to obtain optimal healing. Overall very nice presentation and appreciate for the surgical warnings!
ReplyDeleteGreat job Dan, to answer the questions presented earlier in the comments, I believe that all 3 of the biologics are designed to work together to promote healing. This would be the ideal situation to correct an achilles tendon tear; However, if one had to be omitted, I tend to agree with Dakota's point. Based on the data, PRP would be the best to be removed leaving collagen and MSC. I am curious to see that if one biologics is removed, if wound healing would halt, or just be slowed.
ReplyDeleteEliminating one of the biologics would probably decrease wound healing since his data was comparing the biologics to no biologics. This is a really cool study since achilles tendon injuries are common and take a long time to heal leaving the person to get more atrophy and more physical therapy needed for rehabilitation after the injury.
ReplyDelete