This blog is devoted to BIOL 6988, a graduate level seminar in the biological sciences at Youngstown State University. While targeted towards graduate students, BIOL 6988 actively incorporates undergraduate participants in their scholastic endeavors in the biological sciences. This blog is intended as a educational tool not just for YSU students and faculty, but for anyone who wishes to contribute to an active-learning environment.
S. aureus, as Isaac explained, has become a persistant pathogen in both hospital and community settings. Antibiotic resistant strains (such as MRSA), toxin production to evade the host immune response, and it's capsule promotes survival in the host. However, using phage-display technologies may provide an effective method of attack on S. aureus infections. Using the phage-display cycle, Isaac was able to bio-pan, purify, and sequence the MMT5-4 phage clone specific to the S. aureus capsule, rather than to the whole bacteria. From the sequence, he was able show that MMT5-4 had the same charge and polarity properties described in literature. An amazing presentation by Issac! However, My question to you all, with Issac's explanation of the benefits of phage-display technology, do you think that phage-display can help combat the generation of new antibiotic resistant pathogens? In other words, do you think it could provide an equally effective alternative to antibiotic use?
Its really nice learning new things in every presentation. Big up Isaac! Phage-display has become a common molecular biology technique for the identification of specific peptides and antibodies against pathogen targets. So, its easier to acquire antibodies recognizing different antigen epitopes. I believe since phage-display pans random peptide libraries against a specified target for the identification of linear epitopes that can effectively mimic the epitope structures present in antigen, it can help in fighting against upcoming resistant pathogens. Phage-display can also identify small peptides that bind to pathogens, pathogen membrane proteins and toxins and these small peptides have shown to inhibit the pathogen growth. The biological limitations that impact phage display technology is the well-balanced phage–host system as a whole. The phage–host system is perturbed and may result in anything from a negligibly small alteration in phage growth rate to a complete halt of phage production. Thus the technology has to be used in combination with other methods to realize its effectiveness.
An interesting presentation there Isaac! Yes, I do think Phage display technology can help combat the generation of new antibiotic resistant pathogens. Some studies have shown development of phages with a broader host-range with others engineering pathogenic bacteria in situ using phages so that they can inhibit their ability of transmitting antibiotic resistance determinants.
Great job Isaac! Phage-display is becoming more popular and studied all around the world which marks great opportunity for the future; however, like any other medical treatment, advantages and disadvantages arise. There are many questions that this topic brings to light, but to answer Dan's question, I do believe that phage display technology can provide an equally effective alternative to antibiotics. Although, my question is what would the probability be that bacteria can evolve simultaneously resistant to both phage and antibiotics?
I found this very informative since I have never really had any experience or clue what phage display was or how it could be used. This seems like a cool tool to use for many things. I also like that you included other resources that found different results but both are very important and give more information on how to find a proper solution. I think this could definitely be used as an alternative to antibiotics since it is attacking exactly what it needs to attack and no other bacteria. But what if you have a different bacterial infection then it wouldnt help at all. So give and take. I think it would take a long time for the bacteria to change its dna or rna to resist the phage.
Very interesting and informative presentation by Isaac! I think that phage technology could be used instead of antibiotics. It would be beneficial in terms of antibiotic resistant bacteria and for individuals commonly on antibiotic treatments.
S. aureus, as Isaac explained, has become a persistant pathogen in both hospital and community settings. Antibiotic resistant strains (such as MRSA), toxin production to evade the host immune response, and it's capsule promotes survival in the host. However, using phage-display technologies may provide an effective method of attack on S. aureus infections. Using the phage-display cycle, Isaac was able to bio-pan, purify, and sequence the MMT5-4 phage clone specific to the S. aureus capsule, rather than to the whole bacteria. From the sequence, he was able show that MMT5-4 had the same charge and polarity properties described in literature. An amazing presentation by Issac! However, My question to you all, with Issac's explanation of the benefits of phage-display technology, do you think that phage-display can help combat the generation of new antibiotic resistant pathogens? In other words, do you think it could provide an equally effective alternative to antibiotic use?
ReplyDeleteIts really nice learning new things in every presentation. Big up Isaac!
ReplyDeletePhage-display has become a common molecular biology technique for the identification of specific peptides and antibodies against pathogen targets. So, its easier to acquire antibodies recognizing different antigen epitopes. I believe since phage-display pans random peptide libraries against a specified target for the identification of linear epitopes that can effectively mimic the epitope structures present in antigen, it can help in fighting against upcoming resistant pathogens. Phage-display can also identify small peptides that bind to pathogens, pathogen membrane proteins and toxins and these small peptides have shown to inhibit the pathogen growth. The biological limitations that impact phage display technology is the well-balanced phage–host system as a whole. The phage–host system is perturbed and may result in anything from a negligibly small alteration in phage growth rate to a complete halt of phage production. Thus the technology has to be used in combination with other methods to realize its effectiveness.
Annah Oigo
DeleteAn interesting presentation there Isaac! Yes, I do think Phage display technology can help combat the generation of new antibiotic resistant pathogens. Some studies have shown development of phages with a broader host-range with others engineering pathogenic bacteria in situ using phages so that they can inhibit their ability of transmitting antibiotic resistance determinants.
ReplyDeleteGreat job Isaac! Phage-display is becoming more popular and studied all around the world which marks great opportunity for the future; however, like any other medical treatment, advantages and disadvantages arise. There are many questions that this topic brings to light, but to answer Dan's question, I do believe that phage display technology can provide an equally effective alternative to antibiotics. Although, my question is what would the probability be that bacteria can evolve simultaneously resistant to both phage and antibiotics?
ReplyDeleteI found this very informative since I have never really had any experience or clue what phage display was or how it could be used. This seems like a cool tool to use for many things. I also like that you included other resources that found different results but both are very important and give more information on how to find a proper solution. I think this could definitely be used as an alternative to antibiotics since it is attacking exactly what it needs to attack and no other bacteria. But what if you have a different bacterial infection then it wouldnt help at all. So give and take. I think it would take a long time for the bacteria to change its dna or rna to resist the phage.
ReplyDeleteVery interesting and informative presentation by Isaac! I think that phage technology could be used instead of antibiotics. It would be beneficial in terms of antibiotic resistant bacteria and for individuals commonly on antibiotic treatments.
ReplyDelete